Fig. 5

ASK1 cKO affected polarization of Mi/Mϕ in the hippocampus on day3 after KA administration. A, B Representative images of pro-inflammatory Mi/Mϕ (CD16/Iba1, red/green) A or anti-inflammatory Mi/Mϕ (CD206/Iba1, red/green) B immunostaining in the CA1, CA3, and DG regions of the hippocampus. Scale bar = 50 µm. C Quantification of the total number of Iba1⁺ cells. D Three-dimensional reconstruction of representative pro- and anti-inflammatory Mi/Mϕ was performed with Imaris 9.0.1 software. Scale bar = 10 µm. E–G Quantification of CD16⁺/Iba1⁺ or CD206⁺/Iba1⁺ expression in the CA1(E), CA3 (F), and DG G region. All data are expressed as the mean ± SD.***p < 0.001, **p < 0.01, and *p < 0.05, ns:no significance, as indicated. n = 5/group. p values are based on the one way ANOVA (C), Student’s t test(E–G)