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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Conditional knockout of ASK1 in microglia/macrophages attenuates epileptic seizures and long-term neurobehavioural comorbidities by modulating the inflammatory responses of microglia/macrophages

Fig. 7

ASK1 cKO increased the Mi/Mϕ distribution close to apoptotic neurons 3 day after KA injection. A Representative images of Iba1 (green) and CD68 (grey) immunofluorescence staining in the hippocampus. Scale bar = 30 µm. B Quantification of the mRNA levels of C3 and CD68, n = 4 in the WT sham group, n = 7 in the WT KA group. C Representative images of TUNEL (red) staining, NeuN(green) and CD68 (grey) immunostaining in the hippocampus (blue staining indicates DAPI). Scale bar = 30 µm. Three-dimensional reconstruction of representative CD68+/neun+ /TUNEL+ cells by Imaris 9.0.1 software. D Illustrates the proximal area and distal area. E Quantification of the Mi/Mϕ density in the proximal, distal area. F Quantification of enwrapping (NeuN+ TUNEL+ CD68+) Mi/Mϕ in proximal area. Small plots of the yellow square boxes (a–f) were zoomed to show "Enwrap" state of CD68+ cells. All data are expressed as the mean ± SD. ***p < 0.001, **p < 0.01, and *p < 0.05, as indicated. n = 5 in each group. p values are based on the Student’s t test (B, E, F)

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Journal of Neuroinflammation

ISSN: 1742-2094