Fig. 2

ISP promotes long-term sensory-motor behavioral recovery following ICH. a Irregularly spaced horizontal ladder device was used to assess paw placement accuracy during walking. b, c At week 1 post-ICH, the affected forelimbs of mice in the vehicle and ISP groups had similar error ratios and running scores. However, from week 3 post-ICH onward, ISP-treated mice exhibited progressive and rapid recovery compared to the vehicle group in their error ratios and score. d Transparent cylinder was adapted to assess affected forelimb use and asymmetry. e Similar reduction was observed in the asymmetry score of affected forelimbs during rearing in both vehicle- and ISP-treated mice 1-week post-ICH. At week 3, ISP-treated mice showed a progressive restoration of the use of the affected forelimb compared to the vehicle treatment group. Vehicle and ISP groups, n = 15–17 per group; Sham group, n = 5. f Mice are placed individually on a mesh floor and applied with acetone. g Throughout the experiment, ISP treatment did not induce hypersensitivity of any limbs in all three groups when assessed by the acetone test. Vehicle and ISP groups, n = 9–10 per group; Sham group, n = 5. Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparisons test, and expressed as mean ± SEM. ***p < 0.001; ns, not significant