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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Macrophages in close proximity to the vitreoretinal interface are potential biomarkers of inflammation during retinal vascular disease

Fig. 4

Perivascular macrophages are discrete from microglia. High resolution confocal immunofluorescence of CD31 (blue), GFP (green), and IBA1 (red) from Tmem119GFP/+ retinal flatmounts. For all panels, the main image is the XY for a Z-plane defined by the gray line in both the YZ plane to the right of the main image and the XZ plane to the bottom. A–C Z-stack series from just below the retinal surface (A) to the superficial level (C) demonstrated the difference in ramifications between microglia (yellow/green, A, B) and perivascular macrophages (red, C). The second identically stained series (D–F) showed the distinction between deeper microglia (green/yellow, D), perivascular macrophages (red, adjacent to blood vessel, E), and a third macrophage on the bottom right away from the blood vessel (red, F). Scale bars are 10 microns. Representative image from 3 independently imaged mice from a single litter

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