Fig. 4From: Macrophages in close proximity to the vitreoretinal interface are potential biomarkers of inflammation during retinal vascular diseasePerivascular macrophages are discrete from microglia. High resolution confocal immunofluorescence of CD31 (blue), GFP (green), and IBA1 (red) from Tmem119GFP/+ retinal flatmounts. For all panels, the main image is the XY for a Z-plane defined by the gray line in both the YZ plane to the right of the main image and the XZ plane to the bottom. A–C Z-stack series from just below the retinal surface (A) to the superficial level (C) demonstrated the difference in ramifications between microglia (yellow/green, A, B) and perivascular macrophages (red, C). The second identically stained series (D–F) showed the distinction between deeper microglia (green/yellow, D), perivascular macrophages (red, adjacent to blood vessel, E), and a third macrophage on the bottom right away from the blood vessel (red, F). Scale bars are 10 microns. Representative image from 3 independently imaged mice from a single litterBack to article page