Fig. 8

CCL2 injection increased Ly6C⁺ inflammatory cells on the retinal surface. Superficial confocal immunofluorescence images of Tmem119^GFP/+ retinal flatmounts stained with CD31 (blue), Ly6C (red), GFP (green), and IBA1 (magenta). Representative image from control retina (A–D) showed no Ly6C⁺ cells. Representative CCL2-treated mouse demonstrated a dramatic increase in Ly6C⁺ cells at the retina surface (E–H). Representative image from 3 control and 3 CCL2-treated mice from a single experiment from 2 litters of mice