Fig. 2
From: Sensory neuron dysfunction in orthotopic mouse models of colon cancer
Animal well-being- and pain-related behavior tests in the MC38 colon cancer model. a Nestlet shredding assay. Columns depict percentage (by weight) of nestlet shredded after the 3 h experimental time period. No change in the nestlet shredding indicates proper physiological behavior (p = 0.8, two-tailed unpaired t test). b Weight gain (g) of vehicle vs MC38-injected mice after inoculation (compared to baseline at week 0, before injection). Data show no significant difference in the growth of mice with or without colon cancer (mixed-effects analysis with Å Ãdák's multiple comparisons test). c Changes in abdominal sensitivity of vehicle vs MC38-injected mice after inoculation. The graph shows the scored response provoked by the application of the 0.02 g Von Frey filament to the abdomen (mixed-effects analysis with Å Ãdák's multiple comparisons test). d Mechanical sensitivity of hind paws, as measured by the application of von Frey filaments, by the up-and-down method. Datapoints represent the average of percentage changes, as compared to the measured individual baseline values (measured at week 0, before inoculation). Animals did not develop significant mechanical allodynia (mixed-effects analysis with Å Ãdák's multiple comparisons test). e Changes in the removal of adhesive tape from the plantar surface of the hindpaw, depicted as latency to contact (s), and latency to removal (s), respectively. Animals with colon cancer did not develop elevated or diminished tactile sensitivity (mixed-effects analysis with Å Ãdák's multiple comparisons test). f Cold sensitivity of hind paws. Withdrawal threshold: time until sudden removal of the hind paw (s). The induction of colon cancer did not result in elevated cold sensitivity in the experimental period (mixed-effects analysis with Å Ãdák's multiple comparisons test)