Fig. 1

High levels of chimerism (> 78%) were observed 4 weeks post-bone marrow transplantation (BMT). Male C57BL/6J mice were administered with busulfan (30 mg/kg) for 2 consecutive days, and then 5 × 10⁶ GFP⁺ BM cells were injected into the tail vein of recipient mice. The peripheral blood was collected 4 weeks post BMT and GFP⁺ cells were quantified. A Schematic drawing of the experiment schedule. B BMT process. C Representative picture of peripheral blood cytospin slides (n = 4 mice from the control group). D Representative flow cytometric data of GFP/side scatter (SSC) of peripheral blood (n = 4 mice from the control group). Scale bars, 50 μm. GFP, green fluorescent protein. DIC, differential interference contrast