Fig. 2

CPS induced BM-derived monocyte migration into the hippocampus and differentiation into microglia-like cells. A Schematic of the experimental schedule. Mice received BMT treatment at 6 weeks old, and after 4 weeks, they were exposed to CPS for 5 consecutive days. B Schematic representation of CPS treatment (360 trials within 60 min, intensity of 0.2 mA, variable duration of 1–5 s, and variable intervals of 1–15 s). C Areas in the red rectangle show the region of the hippocampus in which the representative images were taken. D Representative images of GFP⁺ cells (green) in the hippocampus. Scale bars, 200 μm. E Quantification of GFP⁺ cells recruited to the hippocampus (n = 5 mice/group, Mann–Whitney U test, P = 0.008). Graphical data are represented as box and whisker plots. The box shows the lower, median and upper quartiles, and the whisker shows the minimum and maximum values. F GFP⁺ cells (green) overlapped with Iba1 (red) (n = 3 mice/group). Scale bars, 50 μm. **P < 0.01