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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Psychological stress induces depressive-like behavior associated with bone marrow-derived monocyte infiltration into the hippocampus independent of blood–brain barrier disruption

Fig. 3

CPS does not affect the integrity of the BBB. A qPCR analysis showed no difference in mRNA expression of tight-junction proteins between the two groups (n = 4 mice/group, unpaired two-sided Student’s t test, P > 0.05). B Representative western blots showed no difference in the expression of tight-junction proteins between the two groups (n = 3 mice/group). C Quantitative analysis of tight-junction proteins. Data were normalized to GAPDH expression and expressed as fold change (n = 3 mice/group, unpaired two-sided Student’s t test, P > 0.05). D Representative immunofluorescence images of freshly isolated hippocampal microvessels showed intact and continuous staining of Occludin and ZO-1, and no difference was observed between the two groups (n = 5 mice/group). Scale bars, 25 μm. E Quantification of the immunofluorescence intensity of Occludin and ZO-1 (n = 5 mice/group, unpaired two-sided Student’s t test, P > 0.05). a.u., arbitrary units. F Representative images from the hippocampus in which no extravasation of Evans blue was detected in the control and CPS-treated groups compared with the positive control group treated with LPS (n = 3 mice/group). White arrows in the positive control group indicate the visible extravasation of Evans blue (Evans blue displays blue color or red color under bright-field or laser beams, respectively). Scale bars, 200 μm. Error bars represent SEM

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