Fig. 3

Effects of FFA on neuropathological damage after CA/CPR. A Representative photomicrographs of post-cardiac arrest neuropathological damage characterized by immunofluorescence staining for NeuN, MAP2, Iba1, and GFAP in the hippocampal CA1 region of the sham group and the experimental groups at 7 days after ROSC. Scale bar, 100 μm or 50 μm. B Semi-quantitative results of NeuN, MAP2, Iba1, and GFAP staining. Representative photomicrographs of Fluoro-Jade C (FJC) staining (C) and terminal deoxynucleotide transferase-mediated dUTP-biotin nick-end labeling (TUNEL) staining (D) in the cerebral cortex, striatum, and hippocampal CA1 region of the sham group and the experimental groups at 3 days after ROSC. Scale bar, 100 μm. E, F Quantification of FJC-positive and TUNEL-positive cells, respectively. ***P < 0.001 versus the sham group; ##P < 0.01, ###P < 0.001 versus the vehicle group, n = 4–7