Fig. 5

Effects of FFA on BBB integrity after CA/CPR. A Representative photomicrographs of immunofluorescence staining with IgG antibody (red) in the cerebral cortex, striatum and hippocampus of the sham group and the experimental groups at 3 days after ROSC. Scale bar, 100 μm or 50 μm. B Representative photomicrographs of immunofluorescence staining for tight junction proteins (green), including Occludin, Claudin-5, ZO-1, and CD31 (red) with DAPI (blue) in the brains of the sham group and the experimental groups at 3 days after ROSC. Scale bars, 10 μm. C Semi-quantitative results of IgG leakage. D Quantitative analysis of the Occludin/CD31 ratio, Claudin-5/CD31 ratio and ZO-1/CD31 ratio. E Western blot analysis of tight junction proteins in the sham group and the experimental groups at 3 days after ROSC. F Quantification of Evans blue leakage in the sham group and the experimental groups at 3 days after ROSC. **P < 0.01, ***P < 0.001 versus the sham group; #P < 0.05, ##P < 0.01 versus the vehicle group, n = 4–5