Fig. 6

Effects of FFA on microglia/macrophage polarization after CA/CPR. A Representative photomicrographs of immunofluorescence staining for CD16/32 (green), Arg1 (green) and Iba1 (red) in the brains of the sham group and the experimental groups at 3 days after ROSC. Scale bar, 50 μm. B Quantification of the percentage of CD16/32 and Iba1 double-positive cells as well as Arg1 and Iba1 double-positive cells. C qPCR results of pro-inflammatory and anti-inflammatory markers in the brains of the sham group and the experimental groups at 3 days after ROSC. Western blot analysis of pro-inflammatory and anti-inflammatory markers in the brains of the sham group and the experimental groups at 3 days after ROSC (D) and the associated quantitative graph (E). F Representative photomicrographs of immunofluorescence staining for Iba1 (green) and NeuN (red) in the brains of the sham group and the experimental groups at 3 days after ROSC. Scale bar, 50 μm. G Quantification of the number of Iba1 and NeuN double-positive cells. H Representative photomicrographs of immunofluorescence staining for ICAM-1 in the brain of the sham group and the experimental groups at 3 days after ROSC. Scale bar, 100 μm. I Semi-quantitative results of ICAM-1 staining. *P < 0.05, **P < 0.01, ***P < 0.001 versus the sham group; #P < 0.05, ##P < 0.01, ### P < 0.001 versus the vehicle group, n = 4