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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: IL-27 shapes the immune properties of human astrocytes and their impact on encountered human T lymphocytes

Fig. 2

IL-27 enhances the secretion and surface expression of several immune molecules by untreated or IL-1β-exposed human astrocytes. Untreated (untr) and IL-1β-treated astrocytes (inf) were exposed to IL-27 (100 ng/mL) for 24 h. Supernatants were tested for the secretion of A CXCL9, CXCL10, CXCL11, and B IL-18BP by ELISA. CE Astrocytes were analyzed for surface expression of HLA-ABC, HLA-E, PD-L1, and ICAM-1 using flow cytometry. C Representative histogram of HLA-ABC detection and geometric mean fluorescence intensity are shown. D Representative dot plots for expression of HLA-E, PD-L1, and ICAM-1. E Percentage of positive astrocytes for HLA-E, PD-L1, and ICAM-1. AE Each dot represents one donor. Paired Friedman test (A) or one‐way paired ANOVA followed by Fisher's LSD test. B, C, E comparing Untr and Inf astrocytes in the presence vs. absence of IL‐27 from each group. n = 6–14, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001

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