Fig. 4

T lymphocytes modify their expression of surface immune molecules upon contact with IL-27-treated astrocytes. CD4⁺ and CD8⁺ T lymphocytes alone or co-cultured with untreated, IL-27 or IL-1β-treated astrocytes for 24 h were analyzed by flow cytometry. A Representative dot plots of CD4⁺ T lymphocytes in each condition for the detection of CD95, IL-18Rα, CXCR3, PD-1, PD-L1, and ICAM-1. B–G Percentages of positive CD4⁺ (red) and CD8⁺ T lymphocytes (blue) expressing. B CD95, C IL-18Rα, D CXCR3, E PD-L1, F PD-1, and G ICAM-1 are shown as mean ± SEM. Each dot represents one donor. Paired One‐way ANOVA followed by Fisher's LSD test for comparison between conditions. P values comparing T lymphocytes alone (empty bars) and T cells co-cultured with untreated or treated astrocytes are shown as pink stars. P values for comparison within T lymphocyte–astrocyte co-culture groups are shown as black stars. n = 6–17; *P < 0.05, **P < 0.01, ***P < 0.001 and ***P < 0.0001