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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: IL-27 shapes the immune properties of human astrocytes and their impact on encountered human T lymphocytes

Fig. 5

MS patients’ CD8+ T lymphocytes exhibit higher motility than HC’s upon contact with IL-27-exposed astrocytes. Orange CMRA-labeled astrocytes were treated or not with IL-27 for 24 h. αCD3/αCD28 activated human CD8+ T lymphocytes from healthy control (HC) and MS patient (MS) were added to washed astrocytes, and cells were imaged every minute for 2 h. A Three-dimensional time-lapse view at T = 0, T = 1 and T = 2 h of activated CD8+ T lymphocytes (green) from one MS donor co-cultured on untreated or IL-27-treated astrocytes (magenta). B Box plot presenting the speed mean (µm/min) and arrest coefficient (%) measured for each individual CD8+ T lymphocyte from HC (blue boxes) and MS patients (orange boxes) tracked on untreated or IL-27-treated astrocytes. The line represents the median in each box. Comparisons of CD8+ T lymphocytes from HC and MS patients (pink stars) and T lymphocytes from each group (black stars) are shown. C Proportions of CD8+ T lymphocytes from HC and MS patients exhibiting the scanning, dancing, poking, and round behaviors when co-cultured with untreated or IL-27-treated astrocytes. One‐way ANOVA followed by Fisher's LSD test for comparison between conditions. P values are color-coded for each behavior. DF Box plots presenting the speed mean (µm/min) and the arrest coefficient (%) measured for D scanning, E dancing, F poking CD8+ T lymphocytes from HC (blue boxes) and MS patients (orange boxes) tracked on untreated or IL-27-treated astrocytes. The line represents the median in each box. B, D, E, F Statistical p values for comparisons of CD8+ T lymphocytes from HC and MS patients are shown as black stars, whereas comparisons of T lymphocytes within same donor group are shown as pink stars. Data for astrocyte–CD8+ T cell co-cultures are pooled from 5 distinct experiments, 382 cells from 6 HC and 380 cells from 6 MS patients. Wilcoxon or t-test was used. *P < 0.05, **P < 0.01, ***P < 0.001 and ***P < 0.0001, ****P < 0.00001

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