Table 1 Antibodies for flow cytometry analysis
Targeted human antigen–fluorochrome | Clone | Concentration used per stain | Vendor |
|---|---|---|---|
CD4–BV786 | SK3 | 1 µg/mL | BD Biosciences |
CD8–Super Bright 702 | RPA-T8 | 0.5 µg/mL | eBioscience-Invitrogen |
CD54 (ICAM-1)–BV421 | HA58 | 0.5 µg/mL | BD Biosciences |
CD95 (Fas)–FITC | DX2 | 1.25 µg/mL | BD Biosciences |
CXCR3–BV421 | G025H7 | 20 µg/mL | BioLegend |
PD-1 PE | J105 | 5 µg/mL | eBioscience-Invitrogen |
PD-L1–biotin | MIH1 | 5 µg/mL | eBioscience-Invitrogen |
HLA-ABC Pacific Blue | W6/32 | 40 µg/mL | BioLegend |
HLA-E–PE | 3D12 | 10 µg/mL | ThermoFisher |
IL-18Rα–biotin | H44 | 10 µg/mL | BioLegend |
T-bet–BV421 | O4-46 | 1 µg/mL | BD Biosciences |
EOMES–PE-Cy7 | WD1928 | 0.48 µg/mL | ThermoFisher |
RORγt–Alexa Fluor 647 | Q21-559 | 0.25 µg/mL | BD Biosciences |
GATA-3–PE-cf594 | L50-823 | 0.060 µg/mL | BD Biosciences |
Streptavidin–BV605 |  | 2ug/mL | BD Biosciences |