Fig. 2

Locomotor activity measured by the open field test before and after 6 weeks of intranasal HDM or PBS (control). A, B The ambulatory distance over each 10-min time bin during the 60-min trial before (n = 24, total mice) and after (n = 12 per group) intranasal sensitization. *p < 0.05, comparing exploratory activity over the duration of the session, between treatment groups, two-way ANOVA. C, D A comparison of the total ambulatory distance in individual mice, pre- and post- intranasal sensitization with HDM or PBS (control). Mice in the HDM group did not show a typical reduction of ambulatory distances traveled after sensitization. E, F A comparison of the total time spent in the center of the open field chamber in individual mice (n = 12 each, *p < 0.05, ***p < 0.001, Wilcoxon paired t-test)