Fig. 4

Spatial memory assessed by the Y-maze novel arm test. A Before sensitization, mice spent more time in the novel arm compared to the familiar arm. B After treatment, control mice again spent more time in the novel arm than the familiar arm, however, the HDM-sensitized mice spent about the same time in the familiar arm, indicating poor spatial memory. ****p < 0.0001, **p < 0.01, ns not significant, one-way ANOVA novel vs. familiar. C, D A comparison of the percent time spent in the familiar arm in individual mice. Only mice in the HDM group showed an increased time spent in the familiar arm, again indicating impaired spatial memory. ***p < 0.001, Wilcoxon paired t-test