Fig. 5

IL-27 localization in the retina. a (Upper panels) Immunodetection of IL-27p28 in rd10 mice at age P25. Colocalization of IL-27p28 signal (red) with GFAP-positive processes (green) indicated by asterisks, consistent with expression in Muller glia. Brightness was enhanced in the GFAP panel in order to visualize the processes in the ONL. (Lower panels) Immunodetection of IL-27p28 in primary Muller glia cultures (red). GFAP (green) detection on an adjacent coverslip indicates the purity of the cultures because every cell was GFAP-positive. The “no Ab” panel contains primary Muller glia with no primary antibody at the equivalent exposure settings as the IL-27 image to demonstrate lack of non-specific immunodetection. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. b DNA gel with PCR products showing IL-27 and IL-27Rα amplification in the BV2 mouse microglia cell line, primary Muller glia (MG) and whole retina from rd10 mice