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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model

Fig. 5

miR-26a-5p targets Wnt5a by binding to the 3’-UTR of Wnt5a mRNA. A Bioinformatics analyses of the complementarity of the miR-26a-5p seed sequence to the 3′UTR of Wnt5a and conservation of the putative-binding site in vertebrates. B Luciferase reporter assays in HEK293T cells after co-transfection of cells with the wild-type or mutant 3’-UTR of Wnt5a and the miR-26a-5p, NC mimics or ASO-miR-26a-5p, ASO-NC. C mRNA levels of Wnt5a on day 3, 7 and 14 after surgery. Statistical significance of mean differences was determined with the Tukey’s multiple comparisons test. D, E mRNA(D) and protein(E) levels of Wnt5a in sham, SNI + nc, SNI + miR-26a-5p groups, The SNI + miR group decreased significantly compared with the SNI + NC group, n = 6 mice/group, Data are represented as mean ± sem. **p < 0.01

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