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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Chronic suppurative otitis media causes macrophage-associated sensorineural hearing loss

Fig. 1

Characterization of persister cells (PCs). A Time-dependant survival curve for PA stationary phase after treatment with ofloxacin (5 μg/mL). The survival curve demonstrates rapid killing from 0 to 5 h (predominantly metabolically active cells) and plateauing of survival (PCs) from 5 to 24 h. B Growth curve of ofloxacin-generated PCs compared to stationary phase PA. PCs displayed delayed awakening on growth curve compared to stationary phase PA, consistent with the PC phenotype. Upon awakening, the growth rate of the PC phenotype matches that of the stationary phase PA. C Intracellular ATP level comparison between PCs compared to stationary phase PA. PCs contained significantly less intracellular ATP following normalization to protein levels compared to stationary phase PA. Due to lower metabolic activity, PC exhibit lower ATP level. D The MIC of the surviving PCs was determined by performing growth curves for serial dilutions of ofloxacin (0, 0.48 and 0.96 µg/mL) showing that the PCs are not resistant (MIC < 2 µg/mL)

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