Fig. 2

OHC loss occurs in CSOM. A–C Whole mount immunostaining showed no HC loss.in the base, middle and apex regions of the cochlea at 3 days (A–C), 7 days (D–F) and 14 days (G–I) compared to 14 days control (J–L). The normal condition displays 3 rows of OHCs on the left and 1 row of IHC on the right of the pictures. CSOM cochlea displayed OHC loss at 14 days (G–I) in base (G, arrowheads) and small areas of absence in middle (H, arrowheads), and no OHC in the apex (I). M OHC survival rate was significantly lower in the base (P < 0.001) and middle (P < 0.05) at 14 days compared to control (M). The mouse number for each group is presented in the column (M). Red: myosin VIIa. The data represent mean ± SD. Scale bar = 100 µm