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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Chronic suppurative otitis media causes macrophage-associated sensorineural hearing loss

Fig. 5

Macrophage numbers are significantly increases in the CSOM cochlea. Cochlear cryosections were stained with the macrophage-specific marker F4/80 (green) and were counted in the region of the spiral ligament, the organ of Corti, spiral ganglion neurons, and the scala media as outlined (A-dotted). Myosin VIIa staining (white) labels HCs in the cochlea. Counts were performed in all cochlear turns (base, middle, apex) in control mice (A–C) and CSOM cochleae at 3d (D–F), 7d (G–I), and 14 DAYS (J–L). Arrows in the control group (B) show macrophages in the stria vascularis, spiral ligament and spiral ganglion neuron area. Arrowheads in CSOM at 3d (D) show macrophages also present in the basilar membrane, scala vestibuli. M Statistical analysis of the macrophages per turn, revealed significant macrophage elevation in the CSOM cochlea at all time points compared to control mice. N F4/80-labeled macrophages compared with CD45 positive immune cells showing all similar numbers in all time points except at 3 days. Number of mice per group is in parentheses alongside the timepoint. The data represent mean ± SD. Scale bar = 100 µm

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