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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Chronic delta-9-tetrahydrocannabinol (THC) treatment counteracts SIV-induced modulation of proinflammatory microRNA cargo in basal ganglia-derived extracellular vesicles

Fig. 3

Changes in miRNA expression in BG-EVs from chronically SIV-infected rhesus macaques administered vehicle (VEH) or THC. A–C Volcano plots showing the relationship between fold-change and statistical significance of differentially expressed miRNAs in A VEH/SIV RMs relative to uninfected control, B THC/SIV relative to VEH/SIV RMs, and C THC/SIV relative to control RMs. The blue and red vertical lines correspond to 1.5-fold down and up, respectively, and the horizontal black lines represent p < 0.05. The miRNAs of interest are denoted with arrows. D Heatmap of significant pathways predicted by DIANA-miRPath (v.3) of the human counterparts of the 11 SIV-downregulated miRs, showing inflammation and immune regulation pathway cluster (red arrows), a TLR signaling pathway cluster (orange arrows), in addition to Neurotrophin TRK receptor signaling pathway and response to stress (green arrows), as well as cell death and viral process (black arrows), among others. E Raw counts of selected neuroregulatory miRNAs, downregulated in the VEH/SIV group. F TaqMan PCR validation using mml-let-7a-5p and mml-let-7c-5p specific TaqMan microRNA stem-loop RT-qPCR assays. Statistical differences were assessed by a two-way ANOVA test with Tukey’s correction (n = 3). *p < 0.05

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Journal of Neuroinflammation

ISSN: 1742-2094