Chronic delta-9-tetrahydrocannabinol (THC) treatment counteracts SIV-induced modulation of proinflammatory microRNA cargo in basal ganglia-derived extracellular vesicles

Table 2 Sequencing metrics* across each sample

Sample ID	Raw reads	Trimmed reads (minimum length 5)	Percentage reads (minimum length 5)	Trimmed reads (minimum length 15)	Percentage reads (minimum length 15)	Reads aligned to mmul genome	Percentage reads aligned to mmul genome	Reads aligned to mmul miRBase	Percentage reads aligned to mmul miRBase
IR97	15,040,626	14,916,991	99.2	14,726,019	97.9	5,801,187	39.39	197,985	1.34
IT18	10,553,653	10,383,011	98.4	10,123,258	95.9	8,449,318	83.46	3,437,862	33.96
GT18	8,502,723	8,407,642	98.9	8,250,430	97.0	7,014,323	85.02	2,224,050	26.96
IV95	12,202,389	11,909,194	97.6	11,402,173	93.4	8,043,460	70.54	180,656	1.58
JD66	13,541,260	13,405,955	99.0	13,232,393	97.7	11,158,775	84.33	593,001	4.48
JR36	9,531,380	9,304,961	97.6	8,964,346	94.1	7,063,324	78.79	353,166	3.94
JI45	11,677,881	11,421,702	97.8	10,918,406	93.5	8,837,853	80.94	3,226,625	29.55
JC85	10,290,459	10,095,165	98.1	9,675,224	94.0	6,639,058	68.62	752,874	7.78
IV90	9,073,363	8,899,837	98.1	8,538,395	94.1	6,826,220	79.95	2,065,222	24.19

*After adapters are trimmed, reads are filtered based on length (5 bp, 15 bp). Filtering reads shorter than 5 bp determines RNA degradation. 15 bp is the minimum length for meaningful alignments

ISSN: 1742-2094