Fig. 6

Captopril treatment suppresses the KA-induced contact between glial cells in the hippocampus. A Representative anti-Iba1 and anti-Gfap double immunostaining indicates that captopril treatment reduces the KA-induced contact between astrocytes and microglia. Scale bar = 5 μm. B Quantification of Gfap (red) signal intensity around microglia (cyan) is shown. n = 10–12 cells from 3 rats/group, ^###p < 0.001, compared to control; ^***p < 0.001, compared to KA, one-way ANOVA followed by Tukey’s post hoc test. Data are expressed as mean ± SE