Fig. 2

In vivo cladribine treatment does not affect the number of inflammatory foci in the brain of EAE mice. Hematoxylin and eosin (H&E) staining of coronal sections comparing inflammatory CNS lesions in vehicle- and cladribine-treated EAE mice. Images were acquired with a Zeiss Axio Scope.A1 (Zeiss, Göttingen, Germany) using tenfold objectives. Exemplary H&E stainings of the ipsilateral hemisphere (A) and the contralateral hemisphere (without stereotactic cytokine injection, B) are shown. Scale bar 100 µm. Cc corpus callosum, Cx cortex, Hip hippocampus, Str striatum. C Representative lesions of the ipsilateral (a) and the contralateral (b) hemisphere are magnified. Scale bar 50 μm. D Histograms showing the quantification of inflammatory lesions in both hemispheres in coronal sections of vehicle- and cladribine-treated EAE mice. Lesions are classified topologically (grey (GM) and white (WM) matter). No statistic difference was obtained following unpaired Mann–Whitney U test (p > 0.05). n = 3, 35 slices per animal were analyzed and the mean per slice and mouse was used for statistical analysis