Fig. 11

TD139 reduces microglia reactivity and delays cone photoreceptor death in focal light induced damage. A Light exposure regimen of focal blue light damage and TD139 administration in Cx3cr1^GFP/+ reporter mice. B Representative fundus image of a focal light lesion of a vehicle-treated mouse 4 days post-light exposure. C Representative images of GFP⁺ microglia in the OPL of retinal flat mounts of naïve and light-exposed mice treated with vehicle or TD139. Scale bar: 50 µm. D Quantification of grid crossing points from GFP⁺ cells. Data show mean ± SEM. Naïve n = 128; Vehicle/TD139: 4d n = 309/428 cells. ***P ≤ 0.001 by ordinary one-way ANOVA followed by Tukey’s multiple comparisons. E Representative images of cone arrestin⁺ cells in retinal flat mounts of naïve and light-exposed mice treated with vehicle or TD139. Scale bar: 50 µm. F Quantification of cone arrestin⁺ cells in the retina. Data show mean ± SEM. Naïve n = 5; Vehicle/TD139: 4d n = 3/5 retinas. *P < 0.05 by Kruskal–Wallis test followed by Dunn’s multiple comparison