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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Genetic targeting or pharmacological inhibition of galectin-3 dampens microglia reactivity and delays retinal degeneration

Fig. 3

Galectin-3 deficiency dampens microglia reactivity in light-exposed mice. A Representative images of Iba-1+ cells in the subretinal space of retinal flat mounts from naïve and light-exposed WT and Lgals-3 KO mice. Scale bar: 50 µm. B Quantification of Iba-1+ area in the subretinal space. n = 15 retinas from individual mice. C Number of Iba-1+ cells in the subretinal space. n = 15 retinas from individual mice. D Relative mRNA expression of pro-inflammatory cytokines in retinas of naïve and light-exposed WT and Lgals-3 KO mice normalized to the reference gene Atp5b. n = 6 retinas except naïve n = 4 (iNos) or n = 3 (Il-6, and Ccl2) retinas from individual mice. E Representative images of Iba-1+ cells in RPE/choroids from naïve and light-exposed WT and Lgals-3 KO mice. Scale bar: 50 µm. F Quantification of Iba1+ area in RPE/choroidal flat mounts. n = 15 RPE/choroids from individual mice. G Number of Iba-1+ cells in the RPE/choroid. n = 15 RPE/choroids from individual mice. H Relative mRNA expression of pro-inflammatory cytokines in RPE/choroids of naïve and light-exposed WT and Lgals-3 KO mice normalized to the reference gene Atp5b. n = 6 RPE/choroids except naïve n = 3 RPE/choroids from individual mice. Data show mean ± SEM. *P < 0.05; **P < 0.01 and ***P ≤ 0.001 by ordinary one-way ANOVA followed by Tukey’s multiple comparisons

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