Fig. 2

The survival and activity of puberty-born neurons. A Immunofluorescence was used to characterize the survival of puberty-born neurons. The BrdU/NeuN assay shows the survival of puberty-born neurons in the DG until PND65; the BrdU/NeuN/c-fos assay shows the active puberty-born neurons. NeuN (green pixels), BrdU (red pixels), c-fos (pink pixels), and DAPI (blue pixels). B The percentage of puberty-born (BrdU/NeuN) neurons in the DG. C The percentage of active puberty-born neurons (BrdU/NeuN/c-fos) in BrdU/NeuN cells. P values were calculated using Student’s t-test. Data are presented as mean ± SEM for each group (n = 4 per group). *p < 0.05