Fig. 6

NAM administration reverses the effects of MS on NAD⁺, Sirt3, protein acetylation, and microglial inflammation. A NAD⁺ levels in the hippocampus (CON + Saline, n = 8; MS + Saline, n = 8; CON + NAM, n = 8; MS + NAM, n = 8). B—D Immunoblots and quantification of Sirt3, acetylated SOD2, and total SOD2 in the hippocampus (n = 6, per group). E Representative immunofluorescence images show the expression of Iba1 (green pixels) and Sirt3 (red pixels) in the DG (n = 4 per group). F Quantitative analyses of the percentage of Sirt3 expression. J Quantitative analyses of the percentage of Iba1 and Sirt3 co-labeling. H Quantitative analyses of the Iba1 + immunostained cells. I Real-time qPCR analysis of TNF-α, IL-1β, and IL-6 in the hippocampus (n = 6 per group). Data are presented as mean ± SEM for each group. *p < 0.05, **p < 0.01, and ***p < 0.001