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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Pregabalin mitigates microglial activation and neuronal injury by inhibiting HMGB1 signaling pathway in radiation-induced brain injury

Fig. 1

Pregabalin inhibited microglia activation and inflammatory response in the RIBI mice. A Timeline illustration of the experimental design. B, C The heat map showed the mRNA changes of the pro-inflammatory factors in the hippocampus of mice on day 3 and 7. D–G Q-PCR analysis of Il-1β, Il-6, Tnf-α, and Cox-2 mRNA levels in the cortex of RIBI mice 3 and 7 days after pregabalin treatment. H Representative images of IBA1 and CD68 co-labeling in the cortex of RIBI mice 14 days after pregabalin treatment. I, J Microglial body size and the proportion of CD68+ area/IBA1+ area in the cortex and hippocampus (CA1 and DG) were quantified among the different groups. Data were analyzed by one-way ANOVA followed by the Student’s t-test analysis. All other groups were compared with the indicated group. n = 4–6 mice per group for the heat map analysis. n = 7 mice per group for Q-PCR analysis. n = 4 mice per group and 2–3 slices per mouse for immunofluorescence staining. Data were presented as mean ± SEM, *p < 0.05, **p < 0.01, and ***p < 0.001. Four groups were established for in vivo study: control mice (Con), control mice treated with pregabalin (PGB), RIBI-model mice (Rad), and RIBI-model mice treated with pregabalin (Rad + PGB)

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