Fig. 7

Pregabalin inhibited microglial inflammatory response through neuronal HMGB1 signaling pathway. A, B Immunofluorescence staining was used to detect microglial IL-6 and TNF-α expressions under different conditions in the co-culture system of neurons and microglia. C, D Statistical analysis of IL-6 and TNF-α expressions among the different groups. Data were analyzed by one-way ANOVA followed by the Student’s t-test analysis. All other groups were compared with the indicated group. n = 4 per group for immunofluorescence staining in vitro. Data were presented as mean ± SEM, ns = not significant, *p < 0.05, **p < 0.01, and ***p < 0.001