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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: β-Arrestin2-biased Drd2 agonist UNC9995 alleviates astrocyte inflammatory injury via interaction between β-arrestin2 and STAT3 in mouse model of depression

Fig. 1

CSDS induces astrocyte dysfunction and inflammatory microenvironment in the hippocampus of mice. A Schematic diagram of the model of CSDS. Behavior test of SI ratio (B), sucrose preference test (C), forced swim test (D), and tail suspension test (E) were detected (Control group: n = 12; Susceptible group: n = 16; Resilient group: n = 10). F Immunofluorescence staining the marker of astrocytes (GFAP) in the hippocampus and 3D reconstruction. Red, GFAP; Blue, Hoechst. Scale bar, 50 μm. Enlarge vision, Scale bar, 40 μm. G Schematic diagram of isolating ACSA-2 positive astrocytes from the hippocampus of control and susceptible mice. H Purity of astrocytes isolated by ACSA-2 was identified with flow cytometry. I Heatmap of the RNA sequencing performed in WT and CSDS susceptible mice. J Cytokines and receptors of cytokines such as the interleukin family were listed in susceptible mice compared with controls. Levels of cytokines such as TNF-α, IL-6, IFN-β, and IL-1β were detected by qRT-PCR (K) and ELISA (L). K n = 6; L n = 8. Data are analyzed using one-way ANOVA, then combined with Dunnett’s to assess the differences between groups. ns, P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001

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