Fig. 3

Erbin deficiency facilitates NLRP3 inflammasome activation and pyroptosis of microglia in vivo. A Protein levels of Iba-1, NLRP3, Caspase-1 P20, and GSDMD-N in the hippocampus were measured. B-E Numbers of Caspase-1/Iba1 and GSDMD/Iba1 positive cells in CA1 in the hippocampus were measured by double immunofluorescence staining. Scale bar, 50 μm. F-I IL-1β, IL-18, TNF-α, and IL-6 were measured in the hippocampus by ELISA. Data are representative of three independent experiments. *p < 0.05, **p < 0.01 vs. Sham group; ^#p < 0.05, ^##p < 0.01 vs. CLP group