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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Erbin protects against sepsis-associated encephalopathy by attenuating microglia pyroptosis via IRE1α/Xbp1s-Ca2+ axis

Fig. 4

Erbin deficiency facilitates NLRP3 inflammasome activation and pyroptosis of microglia in primary microglia. A Protein levels of Erbin, Iba-1, NLRP3, Caspase-1 P20, and GSDMD-N in primary microglia were measured by western blot. B, C Fluorescence intensity and morphological changes of primary microglia were observed by confocal microscope. Scale bar, 8 μm. D–G IL-1β, IL-18, TNF-α, and IL-6 levels in primary microglia supernatant were measured by ELISA. H Cell viability of primary microglia was examined by CCK8. I Relative LDH release was detected by LDH kits in primary microglia. J Synapse-related proteins PSD95 and SYP in HT22 cells were measured by western blot. Data are representative of three independent experiments. *p < 0.05, **p < 0.01 vs. Control group; #p < 0.05, ##p < 0.01 vs. LPS/nigericin group

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