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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Erbin protects against sepsis-associated encephalopathy by attenuating microglia pyroptosis via IRE1α/Xbp1s-Ca2+ axis

Fig. 5

Erbin regulates Ca2+ mobilization in primary microglia and BV2 cells. A Fluo4-AM was used to indicate the changes of intracytoplasmic Ca2+ of primary microglia by flow cytometry. B ER Ca2+ of primary microglia located by ER-targeted plasmid CMV-ER-LAR-GECO1 were observed by flow cytometry. C, D MFI of intracytoplasmic Ca2+ and ER Ca2+ in primary microglia were analyzed by flow cytometry. E Concentrations of intracytoplasmic Ca2+ in BV2 cells were observed by confocal microscopy. Scale bar, 25 μm. F Concentrations of ER Ca2+ in BV2 cells were observed by confocal microscopy. Scale bar, 5 μm. G Three main ER stress sensors, ATF6, PERK, and IRE1α, were measured by western blot in primary microglia. H Added STF083010, expressions of Erbin, IRE1α, p-IRE1α and Xbp1s in primary microglia were analyzed by western blot. Data are representative of three independent experiments. *p < 0.05, **p < 0.01 vs. Control group; #p < 0.05, ##p < 0.01 vs. LPS/nigericin group

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