Fig. 7

Erbin regulates pyroptosis via IRE1α/Xbp1s/Ca²⁺ in vivo and in vitro. A Western blot for pyroptosis-related proteins in primary microglia. B–E IL-1β, IL-18, TNF-α, and IL-6 release in the supernatants of primary microglia were measured using the ELISA method. F–G Cell viability and relative LDH release were detected by CCK8 and LDH kits in primary microglia. H–K Double immunofluorescence staining was used to observe the numbers of Caspase-1/Iba1 and GSDMD/Iba1 positive cells in the CA1 area of the hippocampus. Scale bar, 50 μm. L–O IL-1β, IL-18, TNF-α, and IL-6 levels were measured in the hippocampus by ELISA. Data are representative of three independent experiments. *p < 0.05, **p < 0.01 vs. Control or Sham group; ^#p < 0.05, ^##p < 0.01 vs. LPS/nigericin or CLP group; ^&p < 0.05, ^&&p < 0.01 vs. Erbin^−/− + LPS/nigericin or Erbin^−/− + CLP group