Fig. 1
Aβ1–42 i.c.v-induced cognitive impairment and neuronal impairment in mice. A Experimental design. Male C57BL/6 J mice aged 10 weeks were fed for 2 weeks under standard laboratory conditions to acclimate to the environment. At 12 weeks of age, mice were randomly divided into three groups: control group (WT control), sham operation group (WT + PBS), and Aβ1–42 i.c.v group (WT + Aβ). After fecal samples were collected from each group at baseline, surgical administration was performed. At 2 weeks after surgery, feces samples were collected. After 4 weeks, feces samples were collected, and behavioral and pathological detects were performed. B The escape latency from the day1 to day 5. C The swimming track to find the platform on the day 6. D Platform crossing times within 1 min on the day 6. E The escape latency within 1 min on the day 6. F The swimming speed within 1 min. G The swimming distance within 1 min. n = 10 per group. H–I Immunofluorescence and quantification of NeuN in hippocampus (Scale bars, 500 μm) and CA1, CA3, and DG region (Scale bars, 200 μm) of each group (n = 5 per group). Data were presented as mean ± SEM. Statistics were analyzed using one-way analysis of variance (ANOVA) and followed by Fisher’s least significant difference (LSD) test. ∗ P < 0.05, **P < 0.01, ns: no statistical difference