Fig. 1

Frequencies of live, early apoptosis and late apoptosis/NETosis neutrophils from patients and HC after PMA exposure. A Representative flow cytometry plots and gating strategy to determine the frequencies of living, early apoptosis, late apoptosis/NETotic neutrophils in samples from AQP4 + NMOSD and MOGAD patients and their matched HC. Arrows indicate cell-death trajectory of NETosis and apoptosis pathway. Quantification of the frequencies of spontaneous (black dots, none) and PMA-induced cell death (red dots) in B AQP4 + NMOSD or C MOGAD patients compared to their corresponding HC. D AQP4 + NMOSD treatment-naïve patients and HC. Data are represented as median and IQR in scatter plot with a bar graph. Each dot represents an individual subject. none (unstimulated-vehicle; none). HC: n = 29; AQP4 + NMOSD: n = 20; MOGAD: n = 9