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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Impaired response of blood neutrophils to cell-death stimulus differentiates AQP4-IgG-seropositive NMOSD from MOGAD

Fig. 2

Images of neutrophils’ response to PMA in HC as well as MOGAD and AQP4 + NMOSD patients. A Living, apoptotic and NETotic neutrophils from patients and HC. DNA (Hoechst 33342) in blue and neutrophil elastase (NE) in green, as well as merged images, are shown for a HC, b MOGAD and c AQP4 + NMOSD, without PMA (top panels in each group; none) and with PMA (lower panels in each group). B Neutrophils were assigned into three categories based on their nuclei morphology: live, apoptosis or NETosis. C Quantification of NETosis in unstimulated none (black dots) and PMA-stimulated neutrophlis (red dots) from MOGAD (left panel) and AQP4 + NMOSD (right panel) patients compared to their corresponding HC. Data are represented as mean ± SEM in scatter plot with a bar graph. Inverted fluorescent microscope at 40 × magnification; scale bar 20 µm. none (unstimulated-vehicle). HC: n = 9; AQP4 + NMOSD: n = 5; MOGAD: n = 4

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