Fig. 2

P2X7 was mainly expressed on microglia, and P2X7-overexpressing transgenic mice had more strokes. Immunostaining of 50 μm free-floating sections of P2X7-EGFP (A, n = 2) transgenic mice illustrated that P2X7 is highly expressed on IbA1-positive (red) cells compared to NeuN-positive (purple) neurons. DAPI (blue) was used to stain the nucleus. For the negative control, see Additional file 1: Fig. S5. B One day after tMCAO surgery, including an occlusion time of 40 min, the P2X7-overexpressing mice had a significantly higher infarct volume and a significantly higher % loss of parenchyma in the ischemic hemisphere. Statistical significance was analyzed by Student’s t test. **p < 0.001. Data are presented as the median ± range of 9 P2X7-overexpressing mice and 10 wild-type littermate controls. Representative TTC staining and representative overlays of stroke volume at day 1 following tMCAO treatment