Blocking P2X7 by intracerebroventricular injection of P2X7-specific nanobodies reduces stroke lesions

Table 2 DAPI-uptake of microglia after icv injection of P2X7 specific nb-B mod

	DAPI negative cells (in %)	DAPI positive cells (in %)
no nb in vivo + no nb ex vivo + no ATP	98.6	1.4
no nb in vivo + 0.5 μg ex vivo + 0.5 mM ATP	83.3	16.7
30 μg in vivo + no nb ex vivo + 0.5 mM ATP + 160 min circulation	61.9	38.1
no nb in vivo + no nb ex vivo + 0.5 mM ATP	10.5	89.5

Functional analysis of P2X7 activation in microglia after icv injection of nbs was tested by checking the ATP-induced DAPI-uptake (Fig. 5C)

ISSN: 1742-2094