Fig. 2

Nerve injury induces the upregulation of Panx 1 in Schwann cells. A The mRNA expression of Panx 1, Panx 2, and Panx 3 within the sciatic nerves of adult CD1 mice (6–8 weeks). B–D Panx 1 mRNA and protein were detected in sciatic nerves at 4 and 14 days post-CCI. E Double-immunofluorescence labeling with Panx 1 (green) and Schwann cells marker S100 β (red) after CCI at 4 and 14 days. Scale bar = 100 μm. F Quantification of Panx 1 immunostaining intensity in the sham group and the CCI groups. All data are mean ± SEM n = 4 mice/group. *p < 0.05, ***p < 0.001 vs. the sham group. One-way ANOVA followed by Dunnett’s test