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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Fig. 3

Injection of Panx 1 blockers reduces CCI-induced mechanical and thermal pain, and Panx 1 expression. A, B Intrasciatic injection of CBX (100 μM) and probenecid (100 μM) reverses CCI-induced mechanical and thermal pain n = 5 ~ 7 mice/group. C, D Sciatic nerve injection of Panx 1 mimetic peptide, 10Panx reduces mechanical pain for 3 days, and thermal pain for 4 days. However, the scrambled peptide had no effect. Arrows indicate the time-point of drug injection n = 7 mice/group. E, F Representative images of Panx 1 immunostaining at 3 and 7 days after injection with PBS or probenecid (Prob) after CCI in sciatic nerves (E) and the DRG (F). Scale bar = 100 μm. G, H Quantification of Panx 1 immunostaining intensity in different groups shown in E and F. n = 4 mice/group. All data are mean ± SEM. Two-way ANOVA followed by Tukey’s multiple comparison test (for behavioral results) or Dunnett’s test. *p < 0.05, vs. vehicle group, ***p < 0.001, vs. sham group and #p < 0.05, vs. PBS group

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