Fig. 4

Specific knockdown of Panx 1 in Schwann cells reduces CCI-induced neuropathic pain. A, B Intrasciatic injection of shRNA Panx 1-AAV at 10 days post-CCI reduces CCI-induced mechanical and thermal pain, compared with vehicle and Ctrl-AAV groups. Arrows indicate the time-point of virus injection. n = 6–7 mice/group. C, D After behavioral tests (at 24 days post-CCI), the expression of Panx 1 was checked among three groups via immunofluorescent staining n = 3–4 mice/group. Scale bar = 50 μm. All data are mean ± SEM. Two-way ANOVA followed by Tukey’s multiple comparison test for behavioral tests. One-way ANOVA followed by Tukey’s multiple comparison test for immunostaining. *p < 0.05, vs. vehicle and Ctrl-AAV groups, **p < 0.001, vs. Ctrl-AAV group and ***p < 0.0001, vs. vehicle group