Fig. 2

Microglial Nhe1 cKO mice increased white matter tolerance to TBI with enhanced oligodendrogenesis and differentiation. a. Representative immunofluorescent images of MBP staining (at same bregma level) showing the cKO mice exhibited increased corpus callosum (CC) thickness at 1 day after sham or CCI procedures. N = 3 for Ctrl sham (1 male, 2 females) and cKO sham (1 male, 2 females); N = 4 for Ctrl TBI (2 males, 2 females) and cKO TBI (2 males, 2 females). Boxed areas indicate locations where zoom-in images (in b and c) were collected. b. Representative images and quantification of Olig2 colocalized with NG2, Ki67, Caspase-3, and H3K9me3 in CC of Ctrl or cKO brains at 3 days post-TBI. N = 4 for Ctrl (2 males, 2 females) and N = 5 for cKO (3 males and 2 females). Scale bar = 10 µm. c. Representative images of APC⁺ mature oligodendrocytes in the CC of Ctrl or cKO brains at 3 days post-TBI. Arrows: APC⁺ mature OLs. Scale bar = 10 µm. d. Colocalization of Olig2⁺ with the APC⁺ mature oligodendrocytes. e. Quantification of APC⁺ mature oligodendrocytes in the CC of Ctrl or cKO brains at 1, 3, and 30 days post-TBI. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001