Fig. 3

Selective deletion of microglial Nhe1 reduced inflammatory responses in cKO mice at 3 days post-TBI. a Representative gating strategy of CD11b⁺CD45^lo microglia and CD11b⁺CD45^hi myeloid cells using flow cytometry. b Percentages of the CD11b⁺CD45^lo microglia and CD11b⁺CD45^hi macrophage populations within live singlet cells of Ctrl or cKO brains at 3 days post-TBI. c, d Expressions of pro- and anti-inflammatory markers within CD11b⁺CD45^lo or CD11b⁺CD45^hi populations. Data are mean ± SEM, N = 6 for Ctrl (3 males, 3 females) and N = 7 for cKO (4 male, 3 females). e Representative images and quantification of GFAP⁺ reactive astrocytes and IBA1⁺ microglia/macrophages in the peri-lesion cortex of Ctrl or cKO brains at 3 days post-TBI. Scale bar = 20 µm. Data are mean ± SEM, N = 6 for Ctrl (3 males, 3 females) and N = 10 for cKO (6 males, 4 females). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001