Fig. 4

Microglial Nhe1 deletion alters pro- and anti-inflammatory transcriptome profiles in microglia/macrophages at 3 days post-TBI. a Bulk RNAseq of CD11b⁺ microglia/myeloid cells isolated from CL and IL hemispheres of Ctrl and cKO mice at 3 days post-TBI. b Unsupervised hierarchical clustering and heatmap illustration of up- and down-regulated genes. c Venn diagram depicting differential gene expression; FDR q value ≤ 0.05, fold change of ≥ 1.2 or ≤ -1.2. d Volcano plots illustrate the gene expression pattern (detected with log2 fold change of ≥ 1.2 and FDR q-value ≤ 0.05). e Enrichment analysis showing significantly altered top canonical pathways using Ingenuity Pathway Analysis software. f, g Scatter plots showing expression of Th1/Th2 pathway and pro- and anti-inflammatory genes presented as normalized counts. Data are mean ± SEM, N = 4 for Ctrl (all males) and N = 3 for cKO (all males). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001