Fig. 1

TBI leads to altered numbers and distribution of DC subsets at the early phase. A Images of unfixed brains from sham and d3-TBI mice. B Images of formalin fixed brains from sham and d3-TBI mice. C FACS plots indicating frequencies of CD11c⁺MHC-Class II^high cDCs in the spleen of Sham, d1-TBI and d3-TBI mice. Data are representative of two independent experiments. D Cumulative frequencies (top) and absolute numbers (bottom) of cDCs in the spleen of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. E FACS plots indicating frequencies of CD8⁺CD11b⁻cDC1, CD8⁻CD11b⁺cDC2 and CD8⁻CD11b⁻ immature cDCs within pre-gated CD11c⁺MHC-Class II^high cDCs in the spleen of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. F–H Relative frequencies (left), overall frequencies (middle) and absolute numbers (right) of cDC1 (F), cDC2 (G) and immature cDCs (H) subsets in the spleen of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. I FACS plots indicating frequencies of PDCA1⁺CD11c^int pDCs and PDCA1⁻CD11c^high cDCs in the spleen of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. J, K Overall frequencies (left) and absolute numbers (right) of pDCs (J) and cDCs (K) in the spleen of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. L FACS plots indicating frequencies of PDCA1⁺CD11c^int pDCs and PDCA1⁻CD11c^high cDCs in the BM of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. M Overall frequencies (left) and absolute numbers (right) of pDCs in the BM of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. N FACS plots indicating frequencies of CD11c⁺MHC-ClassII^high cDCs in the BM of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. O Overall frequencies (left) and absolute numbers (right) of cDCs in the BM of Sham (n = 4), d1-TBI (n = 5) and d3-TBI (n = 5) mice. Data are representative of two independent experiments. All data represent mean ± SEM. Mann–Whitney non-parametric tests were used to assess statistical significance (*P < 0.05, **P < 0.01, *** P < 0.001, **** P < 0.0001)